Antibacterial & Antioxidant Activity of Different Extract of Moringa Oleifera Leaves – an in - Vitro Study

Moringa oleifera is a common plant and known for various medicinal properties. The research work was conducted to investigate the in vitro antimicrobial and antioxidant activity of aqueous, ethanolic and methanolic extract of leaf Moringa oleifera. In-vitro antimicrobial activity was performed by disc diffusion method in MH agar medium and MIC test. The extract showed significant effect on the tested organisms. The methanolic extract showed maximum zone of inhibition against S.aureus (23.6±1.15), whereas aqueous extract showed lowest against B.subtilis (12.0±1.73). Methanolic extract showed maximum relative percentage inhibition against S.aureus (90.34 %) and aqueous extract showed lowest relative percentage inhibition against B.subtilis (27.86 %). Minimum Inhibitory Concentration (MIC) was measured by tube dilution method. Extract showed 2.4 mg/ml for B.subtilis, E.coli and 7.4 mg/ml MIC values for S.aureus. The antioxidant activities of different concentrations of methanol extracts of the leaves were determined by the three assay techniques i.e. DPPH radical scavenging assay, Nitric oxide scavenging assay, and Hydrogen peroxide scavenging assay method. Methanol extract of leaves has shown effective antioxidant activity in all assay techniques. The results obtained in the present study indicate that the leaves of Moringa oleifera are a potential source of natural antibacterial and antioxidants.